ACT Consulting and MonitoringAn essential part of Premium Purity™ is our service offering where we collect, track and supply you with relevant hygiene data and client reports to ensure monitoring of the established targets. Our service also includes introduction and e-learning of the ACT ECA System. Consequently, ACT.Global becomes your consultant and trusted hygiene partner.
Our customer reports are based on scientific data regarding an environment’s level of cleanliness (ATP) and contamination (TPC). These data are collected during our regular service visits, analyzed and compiled in a customer report that is available online for a client.
How ACT.Global measures hygiene
To establish the hygiene level of a specific area, ACT.Global measures two factors: adenosine triphosphate (ATP) and total plate count (TPC). Adenosine triphosphate measurements determine the cleanliness whereas TPC measurements determine the number of microbes. When combined, these two measurements provide accurate data overview of the hygiene level.
Adenosine triphosphate (ATP)
ATP is a complex organic chemical that provides energy to drive many processes in living cells, e.g. muscle contraction, nerve impulse propagation, and chemical synthesis. ATP can be used by microorganisms as fuel for microbial growth.
ATP is quantified by using a luminometer. A molecule is collected from the target surface using a probe and inserted in the measuring device. Inside the probe, ATP reacts with the firefly enzyme luciferase to produce light. The amount of light produced by this reaction is directly proportional to the amount of ATP collected from the surface. ATP is measured in relative light unit RLU and converted into femtomole (fmol).
To measure the ATP level, we use the 3M™ Clean-Trace™ system, a single-use test that contains a swab for the collection of a sample and a luminometer to measure the result.
Total plate count (TPC)
Total plate count, also known as TPC, is a test used to access the number of microbes from a sampling area. To sample TPC, a nutrient medium (agar) is brought in contact with the surface, transferring the microbes to the agar. The agar is incubated, and the number of microbial colonies appearing is used as an indicator for the microbial level on the surface. The agar plates do not specify individual microbial types. TPC are expressed as colony forming units per square centimetre (CFU/cm2).
All TPC samples are incubated under controlled conditions in a professional lab.
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