An essential Premium Purity™ service includes collecting, tracking, and supplying our clients with relevant hygiene data and reports to ensure established targets are achieved.
Our service also includes introduction and education for the ACT ECA System™. This way, ACT.Global is the client’s consultant and trusted hygiene partner.
Our client reports are based on scientific data regarding an environment’s level of cleanliness (ATP) and contamination (TPC). These data are collected during our regular service visits, and then analyzed and compiled in a client report that is available online for clients.
Every report comes with a manageable data overview that reflects the hygiene level of the client’s facilities.
This ensures that the daily cleaning is adequate, the environment is low-microbial, and the established hygiene targets are achieved.
HOW WE Measure Hygiene
To establish the hygiene level of a specific area, ACT.Global measures two factors: adenosine triphosphate (ATP) and total plate count (TPC). Adenosine triphosphate measurements determine the cleanliness, whereas TPC measurements determine the number of microbes. When combined, these two measurements provide accurate data overview of the hygiene level.
Adenosine triphosphate (ATP)
ATP is a complex organic chemical that can be used by microorganisms as fuel for microbial growth.
ATP is quantified by using a luminometer. A molecule is collected from the target surface using a probe and inserted in the measuring device. Inside the probe, ATP reacts with the firefly enzyme luciferase to produce light. The amount of light produced by this reaction is directly proportional to the amount of ATP collected from the surface.
ATP is measured in relative light unit (RLU) and converted into femtomoles (fmol). To measure the ATP level, we use the 3M™ Clean-Trace™ system, a single-use test that contains a swab for the collection of a sample and a luminometer to measure the result.
Total plate count (TPC)
Total plate count (also known as TPC) is a test used to access the number of microbes from a sampling area. To sample TPC, a nutrient medium (agar) is brought in contact with the surface, and the microbes are transferred to the agar.
The agar is incubated and the number of microbial colonies that appear is used as an indicator for the microbial level on the surface. TPC is expressed as ‘colony-forming units per square centimeter’ (CFU/cm2).
All TPC samples are incubated under controlled conditions in a professional lab.
ACT.Global uses five different classifications of zones/risk levels, based on the risk of contamination.
This means that zones, where the risk of contamination in the presence of microbes is low, are classified as class 1, whereas zones, where the presence of microbes are considered hypercritical, are classified as class 5.
|Performance level||Risk level||ATP threshold [fmol]||TPC threshold [CFU/cm²]|
|1||Minimal||≤ 250||≤ 7.5|
|2||Low||≤ 250||≤ 7.5|
|3||Medium||≤ 175||≤ 5|
|4||Critical||≤ 100||≤ 2.5|
|5||Hypercritical||≤ 25||≤ 2.5|